Bioline成立于1992年，總部設在倫敦，英國。的，是一個不斷發展的化公司，開發，生產和市場，簡化，加速和提高生命科學研究的一個專門的生物研究試劑廣泛。Bioline試劑所使用的分子生物學家和其他研究的科學家來自醫療，生物技術和海洋生物對糧食和農業技術以及法醫和環境科學，生命科學家已經在許多領域進行測試，分析和研究，取決于的品質和可靠性。
英國Bioline產品：
品名：5-hydroxymethyl-2'-deoxycytidine-5'-triphosphate Lithium Salt
貨號：BIO-39046
Product Specifications
Batch details:
Pack Size: 25μmol
Concentration: 100mM
Presentation: 1 x 250μl
Batch No: See vial
Storage Conditions:
Hydroxymethyl dCTP can be stored for 24 months at -20°C. Avoid multiple freeze/thaw cycles. For long-term storage, aliquoting is recommended.
Shipping Conditions:
On Dry Ice or Blue Ice
Product Insert
100mM Hydroxymethyl dCTP
Formula:
C10H14Li4N3O14P3
Molecular Weight:
520.9g/mol
Features
• >99% pure by HPLC
• Readily incorporated by standard DNA polymerases
• Hydroxymethylated substrates can be ligated by standard ligases
• DNase, RNase and Nickase free
• Available exclusively from Bioline, manufactured in our purpose-built lab
應用：
• Site-Directed Mutagenesis
• Substitution of dCTP in a wide variety of molecular biology assays
• Structural and activity studies of the restriction/modification systems of different organisms
• Labelling of DNA in vitro
• Methylation studies
• Studies of Hydroxymethylated DNA/protein interaction
Description
Bioline has developed a novel method for producing highly purified Hydroxymethyl dCTP. Using a unique enzymatic synthesis method, Bioline have been able to mimic the biological steps in the synthesis of Hydroxymethyl dCTP from T-even phages. Highly purified Hydroxymethyl dCTP can be used in a number of molecular biological applications.
Hydroxymethyl dCTP can be used as a substrate for several DNA polymerases under conditions that permit the amplification of DNA containing hydroxymethylated cytosine in place of cytosine. Hydroxymethyl dCTPs can be used to discriminate between the different DNA molecules synthesized in one or several PCR cycles. By the use of appropriate enzymes, it is possible to separate the un-hydroxymethylated starting material from the hemihydroxymethylated intermediate （produced by a single primer extension reaction） and from the fully-hydroxymethylated end product.
This ability to generate PCR products in which cytosine is uniformly replaced by hydroxymethylated cytosine can be applied to: （a） forensic DNA analysis, （b） the development of novel strategies for site-directed mutagenesis and （c） the production of DNA fragments resistant to cleavage by a wide range of restriction endonucleases, useful in the generation of cDNA libraries.
